9 лет практического опыта в сфере новейших мировых технологий защиты растений, агрономии и селекции растений. В том числе в сфере разработок новых продуктов и диджитал решений для агропроизводства, а также практический опыт агрономом на крупных хозяйствах США и Украины. Последние 3 года живу и работаю в Германии. Principles and applications of diagnostic techniques in plant protection with a focus on nucleic acid: electrophoresis (discovery of new viruses, detection of viroids, dsRNA in fungi and hypovirulence, electrophoretic karyotypes); nucleic acid hybridization (spot hybridization, RFLP, ASO, macro- and microarrays); PCR (principle, classical end-point PCR, qPCR and digital PCR; ligase chain reaction; isothermal amplification (LAMP, used of nicking enzymes, helicase, abortive transcript); sources of primers; specificity and sensitivity. Miniaturization of diagnostic methods: lab on chip, ultra-fast PCR. Aptamers. Molecular markers: RFLP, RAPD, AFLP, SSR, SNP; application in epidemiology of pathogens, analysis of population structure and genetic diversity, genome mapping, and map-based cloning of resistance genes. Remote detection of plant pathogens: optical methods and VOC analysis. Biotechnology in plant protection: delimitation from traditional technologies; applications of biotechnology to increase genetic diversity in crops; selection for resistance in tissue culture. Genetic engineering in plant protection: inactivation of genes on DNA and transcript levels; introduction of foreign genes into crop plants: techniques, selection markers, safety, acceptance, legal aspects; GM crops in engineering resistance to viruses, pests, herbicides, bacterial and fungal pathogens. Potential for the use of genetically engineered viruses and microorganisms in plant protection. Current concepts and technologies relevant for plant protection research: genome analysis, omics, saturation mutagenesis, high-throughput screening, systems biology. ENVIRONMENTAL FATE OF PESTICIDES - experimental approaches (lab & field) for the investigation of degradation, metabolism and mobility in water, soil and atmosphere - modelling approaches for the prediction of environmental exposure and effects- PESTICIDE EXPOSURE AND RISK ASSESSMENT - policy and principles of risk assessment - hazard assessment - classification of pesticides by hazard, key toxicological studies used in pesticide risk assessment - dose-response evaluation - exposure assessment - operator exposure and monitoring; field exposure studies; exposure modeling; bystander exposure; consumer exposure - risk characterization - acceptability and uncertainty; risk characterization for threshold and non-threshold effects Regulatory Ecotoxicology of pesticides TOXICOKINETICS - absorption, distribution, elimination of xenobiotic compounds - metabolism of xenobiotics BASIC TOXICOLOGY - definitions, targets organs, molecular mechanisms of action - general toxicology - exposure pathways, time scales acute vs. chronic effects, mutagenesis, cancerogenesis, reproductive toxicity, endocrinic effect, toxicity testing methods TOXICOLOGY OF HERBICIDES, FUNGICIDES, INSECTICIDES AND OTHER PESTICIDE GROUPS - herbicides: triazines: glyphosate, glyphonsinate and related compounds - - fungicides differences between carbamates, (insecticidal/fungicidal), chronic effects - toxicology of Insecticides ECOTOXICOLOGY OF PESTICIDES - data requirements - GLP and data quality - toxicity testing - test species selection - edpoint derivation - risk assessment and Refinement options DEVELOPMENT OF PESTICIDES - (hits, lead molecules, strategies, screens, criteria, costs etc.) REDUCTION OF PESTICIDE RISKS - indicator systems of pesticide risks, monitoring of pesticide use, national action plans, novel EU regulations on risk reduction - food Safety: Regulation of MRLs (maximum residue levels; EFSA) - regulation (EC) No 396/2005 - data to be considered - assessment/models Consumer safety - international aspects (Codex, WTO) - regulatory Framework for Pesticide Registration (national/EU/international; ECCO) GENETIC MAPPING - development of genetic linkage maps - analysis of genome structure - comparative analysis of genome structure - genetic mapping of genes for qualitative traits - genetic mapping of genes for quantitative traits - marker assisted backcrossing (whole genome selection) - development of isogenic lines for genes and QTL DEVELOPMENT OF CLOSELY LINKED MARKERS FOR PHENOTYPICALLY TRACEABLE GENES AND QTL - marker assisted selection - map based cloning - analysis of genetic variation - taxonomic investigations DETERMINATION OF GENETIC DISTANCES WITHIN A SPECIES - selection of superior parental combinations for crosses - development of heterotic groups for hybrid breeding - classification of genetic resources in gene banks - variety identification IDENTIFICATION AND VERIFICATION OF ESSENTIALLY DERIVED VARIETIES UNDERSTANDING FUNCTIONAL BIODIVERSITY FOR COMPLEX TRAITS AND TRANSLATING IT INTO OPTIMIZED BREEDING STRATEGIES